All guides
Read straight through, or jump to whatever you need. Each guide stands on its own and links back to anything it assumes.
Foundations
What the instrument measures, and the physics that lets it.
What an SPR Instrument Actually Measures
Refractive index, not binding — and why that distinction decides every experiment you will ever run.
Evanescent Waves and Surface Plasmons
The physics, built up from total internal reflection with no steps skipped.
Instruments, Optics and Fluidics
What the box around the prism has to do, and which design compromises you inherit when you choose one.
Reading Sensorgrams by Eye
The skill that prevents more bad papers than any fitting software ever will.
Kinetics & Analysis
Turning a curve into two rate constants you can defend.
Binding Kinetics and Mass Transport
The 1:1 model derived properly, then the transport problem that quietly invalidates it.
Beyond 1:1 — Heterogeneity, Avidity and Distributions
What to do when the residuals are structured, and why adding model terms is usually the wrong answer.
Surfaces & Practice
Chemistry, experimental design, processing, and what goes wrong.
Sensor Surfaces and Immobilisation Chemistry
Gold, thiols, hydrogels and coupling chemistry — where most SPR experiments are actually won or lost.
Designing an Experiment That Will Work
Every decision made before the first injection, and the reasoning behind each one.
Processing Raw Data and Fitting It Honestly
What to subtract, in what order, and how to tell whether a fit means anything.
Troubleshooting and Artefacts
A symptom-first field guide, organised the way problems actually present themselves.
Applications
Small molecules, antibodies, imaging, and neighbouring techniques.
Small Molecules and Fragment Screening
Where the usual rules have to be renegotiated, and exactly why the reversal is justified.
Antibody Characterisation: Ranking, Binning and Mapping
Three different questions about a panel, three different experiments, and the avidity trap running through all of them.
SPR Imaging, LSPR, BLI and Interaction Networks
What sits next to SPR, how it differs, and where combining techniques is genuinely worth the effort.
Reporting, Reviewing and Reading Critically
A checklist for your own manuscripts, and for the ones you are asked to referee.